Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
eIF4A (C32B4) Rabbit Monoclonal Antibody detects endogenous levels of total eIF4A protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Met316 of human eIF4A protein.
Background
A variety of factors contribute to the important biological event of translation initiation. The eukaryotic initiation factor 4F (eIF4F) complex of translation initiation factors binds to the 5' m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5' mRNA untranslated region to mediate ribosome binding (2,3). In addition, eIF4A has recently been shown to repress Dpp/BMP signaling in a translation-independent manner in (4,5).
Alternate Names
ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-1; ATP-dependent RNA helicase eIF4A-2; ATP-dependent RNA helicase eIF4A-3; BM-010; DDX2A; DDX2B; DDX48; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; DEAD box protein 48; DKFZp686O16189; EIF-4A; eIF-4A-I; eIF-4A-II; eIF-4A-III; EIF4A; eIF4A-I; eIF4A-II; eIF4A-III; EIF4A1; EIF4A2; EIF4A3; eIF4AIII; EIF4F; Eukaryotic initiation factor 4A-I; Eukaryotic initiation factor 4A-II; Eukaryotic initiation factor 4A-III; Eukaryotic initiation factor 4A-III, N-terminally processed; Eukaryotic initiation factor 4A-like NUK-34; eukaryotic initiation factor 4AI; eukaryotic translation initiation factor 4A; Eukaryotic translation initiation factor 4A isoform 3; eukaryotic translation initiation factor 4A, isoform 1; eukaryotic translation initiation factor 4A, isoform 2; eukaryotic translation initiation factor 4A, isoform 3; eukaryotic translation initiation factor 4A1; eukaryotic translation initiation factor 4A2; eukaryotic translation initiation factor 4A3; Fal1; hNMP 265; IF4A1; IF4A2; IF4A3; KIAA0111; MGC10862; MUK34; NMP 265; NMP265; Nuclear matrix protein 265; NUK34; RCPS
