Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:200
Immunofluorescence (Immunocytochemistry): 1:100 - 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
NBR1 (E6Q3F) Rabbit Monoclonal Antibody recognizes endogenous levels of total NBR1 protein. This antibody is suitable for detecting NBR1 by immunofluorescence when it's expressed at moderate-to-high levels.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro597 of human NBR1 protein.
Background
Next to BRCA1 gene 1 (NBR1) protein is known for its encoding gene proximity to the BRCA1 tumor suppressor gene (1,2). N-terminal Phox and Bem1p (PB1) domains of NBR1 mediate its interaction with muscle specific titin kinase (3,4) and scaffolding protein p62 (4). NBR1 plays a role in selective autophagy by facilitating the autophagosomal degradation of ubiquitinated proteins independently and also in concert with p62 (5-11).
Alternate Names
1A1-3B; 1A13B; B-box protein; Cell migration-inducing gene 19 protein; FLJ55359; FLJ98272; IAI3B; KIAA0049; M17S2; Membrane component chromosome 17 surface marker 2; membrane component, chromosome 17, surface marker 2 (ovarian carcinoma antigen CA125); MIG19; migration-inducing protein 19; NBR1; NBR1 autophagy cargo receptor; neighbor of BRCA1 gene 1; Neighbor of BRCA1 gene 1 protein; Next to BRCA1 gene 1 protein; Protein 1A1-3B
