Description
Product Usage Information
Western Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:200 - 1:800
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 37481 .
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
TdTomato (E3G5L) Rabbit Monoclonal Antibody detects transfected and transgenic levels of TdTomato-tagged proteins (either N-terminally tagged or C-terminally tagged). Please note that the TdTomato tags add approximately 54.2 kDa to the molecular weight of the fusion protein.
Species Reactivity: All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of TdTomato protein.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. TdTomato is a fluorescent, photostable orange dye that was isolated from and further engineered from the Discosoma sea anemone (1). Reported to be six times brighter than EGFP, TdTomato is comprised of a tandem dimer of monomers engineered from DsRed; the dimers are held together by intramolecular bonds. For this reason, TdTomato is considered a monomer. Its peak fluorescent excitation is at 554 nm and emission is at 581 nm (1).
