Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
CDCA5 (E7M3N) Rabbit Monoclonal Antibody recognizes endogenous levels of total CDCA5 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe247 of human CDCA5 protein.
Background
Cohesin is a ring-shaped multiprotein complex that functions in sister chromatid cohesion and in establishing higher order chromosome structure. The cohesin complex regulates chromosome segregation, DNA repair, and transcription (1,2). CDCA5 (also known as Sororin) stabilizes the cohesin complex on DNA through inhibition of the cohesion antagonist WAPL (3). CDCA5 promotes cell proliferation and inhibits apoptosis through the AKT signaling pathway in hepatocellular carcinoma (HCC) cells (4). Overexpression of CDCA5 indicates poor prognosis in HCC (5-7).
Alternate Names
CDCA5; cell division cycle associated 5; Cell division cycle-associated protein 5; MGC16386; p35; Sororin
