Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
FoxD3 (D20A9) Rabbit Monoclonal Antibody detects transfected levels of total FoxD3 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues at the C-terminus of human FoxD3.
Background
FoxD3 is a member of the Forkhead Box family and is characterized by a winged-helix DNA-binding structure and the important role it plays in embryonic development (1). This transcriptional regulator is required for the maintenance of pluripotency in the pre-implantation and peri-implantation stages of mouse embryonic development (2) and is also required for trophoblast formation (3). FoxD3 is required for the maintenance of the mammalian neural crest; FoxD3 mouse embryos fail around the time of implantation with loss of neural crest-derived structures (4). FoxD3 also forms a regulatory network with Oct-4 and NANOG to maintain the pluripotency of ES cells (5,6).
Alternate Names
AIS1; forkhead box D3; Forkhead box protein D3; FOXD3; Genesis; HFH2; HNF3/FH transcription factor genesis; VAMAS2
