Description
Product Usage Information
Western Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:100
Immunofluorescence (Immunocytochemistry): 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 74085 .
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
PP2A A Subunit (81G5) Rabbit Monoclonal Antibody detects endogenous levels of PP2A A subunit, alpha isoform. This antibody does not cross-react with other PP2A subunits.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PP2A A Subunit protein.
Background
Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). The core enzyme consists of catalytic C and regulatory A (or PR65) subunits, with each subunit represented by α and β isoforms (1). Additional regulatory subunits belong to four different families of unrelated proteins. Both the B (or PR55) and B' regulatory protein families contain α, β, γ and δ isoforms, with the B' family also including an ε protein. B'' family proteins include PR72, PR130, PR59 and PR48 isoforms, while striatin (PR110) and SG2NA (PR93) are both members of the B''' regulatory protein family. These B subunits competitively bind to a shared binding site on the core A subunit (1). This variable array of holoenzyme components, particularly regulatory B subunits, allows PP2A to act in a diverse set of functions. PP2A function is regulated by expression, localization, holoenzyme composition and post-translational modification. Phosphorylation of PP2A at Tyr307 by Src occurs in response to EGF or insulin and results in a substantial reduction of PP2A activity (4). Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed (5,6). Methylation alters the conformation of PP2A, as well as its localization and association with B regulatory subunits (6-8).
Alternate Names
2AAA; Medium tumor antigen-associated 61 kDa protein; MGC786; MRD36; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha; PP2A-Aalpha; PP2AA; PP2AAALPHA; PPP2R1A; PR65A; protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform; protein phosphatase 2 scaffold subunit Aalpha; protein phosphatase 2, regulatory subunit A, alpha; serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alpha isoform; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; testicular secretory protein Li 1
