Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Centrin-2 Antibody detects endogenous levels of total centrin-2 protein.
Species Reactivity: Human, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human centrin-2. Antibodies are purified using protein A and peptide affinity chromatography.
Background
Centrins are small conserved microtubule nucleating proteins localized to centrosomes, also known as microtubule organizing centers (MTOC), in eukaryotic cells. Centrin-1 is associated with cells that have cilia and flagella, whereas centrin-2 and -3 are ubiquitously expressed and important in centrosome duplication during cell division, as well as the structure and function of the MTOC (1-3). Human centrin-2 has also been shown to localize to nuclear pores and to have a role in regulation of mRNA export (4). The yeast ortholog of centrin-2, CDC31, plays a part in control of protein degradation and sensitivity to DNA damage (5).
Alternate Names
CALT; caltractin (20kD calcium-binding protein); Caltractin isoform 1; CEN2; centrin 2; Centrin-2; centrin, EF-hand protein, 2; CETN2
