Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
NSF Antibody detects endogenous levels of total NSF protein.
Species Reactivity: Human, Mouse, Rat, Hamster, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu524 of human NSF. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). Following membrane fusion, the α-SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the α-SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (3,4). The ATPase activity of NSF induces a conformational change in the α-SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3,4).
Alternate Names
epididymis secretory sperm binding protein; N-ethylmaleimide sensitive factor, vesicle fusing ATPase; N-ethylmaleimide-sensitive factor; N-ethylmaleimide-sensitive factor-like protein; N-ethylmaleimide-sensitive fusion protein; NEM-sensitive fusion protein; NSF; SEC18; SKD2; Vesicle-fusing ATPase; Vesicular-fusion protein NSF
