Description
Product Usage Information
Western Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:100
Immunofluorescence (Immunocytochemistry): 1:50
Flow Cytometry (Fixed/Permeabilized): 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
The alpha/beta-Tubulin Antibody detects endogenous levels of both α- and β-tubulin total protein, and does cross-react with both recombinant α- and β-tubulin.
Species Reactivity: Human, Mouse, Rat, Monkey, Zebrafish, Bovine
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human α- and β-tubulin. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
Alternate Names
alpha tubulin; Alpha-tubulin ubiquitous; beta 5-tubulin; beta Ib tubulin; beta-4 tubulin; CDCBM6; CSCSC1; Detyrosinated tubulin alpha-1B chain; epididymis secretory sperm binding protein; K-ALPHA-1; M40; MGC117247; MGC16435; OK/SW-cl.56; TBA1B; TBB5; TUBA1B; TUBB; TUBB1; TUBB5; tubulin alpha 1b; Tubulin alpha-1B chain; Tubulin alpha-ubiquitous chain; Tubulin beta chain; tubulin beta class I; tubulin beta polypeptide; tubulin beta-1 chain; Tubulin beta-5 chain; Tubulin K-alpha-1; tubulin, alpha 1b; tubulin, alpha, ubiquitous; tubulin, beta; tubulin, beta polypeptide
