Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
PGC-1 alpha (3G6) Rabbit Monoclonal Antibody detects transfected levels of total PGC-1α protein. This antibody does not cross-react with PGC-1β or other related proteins.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human PGC-1α fusion protein.
Background
PPARγ coactivator-1α (PGC-1α) was originally identified as a transcriptional coactivator whose expression closely correlated with adaptive thermogenesis following exposure to cold temperatures (1). Named for its association with the nuclear receptor peroxisome-proliferator activated receptor (PPARγ), PGC-1α interacts with a diverse array of transcription factors to regulate numerous aspects of cell physiology (2). PGC-1α helps to regulate cell processes important in adaptive thermogenesis and energy metabolism, including the related functions of glucose uptake, gluconeogenesis, insulin secretion, and mitochondrial biogenesis (3). Long thought to be a potential therapeutic target for the treatment of type II diabetes, obesity, cardiomyopathy, or other metabolic disorders (reviewed in 4), a recent functional survey found no obvious differences in PPARγ activity associated with recognized PGC-1α variants (5).
Alternate Names
L-PGC-1alpha; LEM6; Ligand effect modulator 6; ligand effect modulator-6; peroxisome proliferative activated receptor, gamma, coactivator 1, alpha; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-3ext; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-8a; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5-NT; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; peroxisome proliferator-activated receptor gamma, coactivator 1 alpha; PGC-1 alpha; PGC-1-alpha; PGC-1(alpha); PGC-1alpha; PGC-1v; PGC1; PGC1 alpha; PGC1A; PPAR gamma coactivator variant form; PPAR gamma coactivator-1; PPAR-gamma coactivator 1-alpha; PPARG coactivator 1 alpha; PPARgamma coactivator 1alpha; PPARGC-1-alpha; PPARGC1; PPARGC1A; PRGC1
