Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
ANTXR1/TEM8 Antibody recognizes endogenous levels of total ANTXR1/TEM8 protein.
Species Reactivity: Human, Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg557 of human ANTXR1/TEM8 protein. Antibodies are purified by peptide affinity chromatography.
Background
ANTXR1 (Anthrax toxin receptor 1, TEM8, tumor endothelial marker 8) is a protein biomarker induced in tumor-associated endothelia and acts as a cell surface receptor for the anthrax toxin and the oncolytic Seneca Valley Virus (1-3). The protein is a type I transmembrane glycoprotein that is expressed on the epithelial cell surface of skin, lung, and intestine tissues as a docking site for toxin/virus binding and endocytosis, and in tumor endothelial cells for tumor-associated vascular development (4). ANTXR1 interacts with type I and type VI collagens in the extracellular matrix, and with actin cytoskeleton in the cytoplasm to promote tumor-associated endothelial cell adhesion, migration, and spreading during angiogenesis (4-6). Aberrant high expression of ANTXR1 has been found in various cancer types including metastatic breast cancer cells, pancreatic cancer stem cells, etc. (7-9); there the protein promotes tumor proliferation, growth, and invasion. Because of its specific high expression in tumors and to their surrounding vasculature, ANTXR1 has been proposed as a target for CAR T cell therapy for cancer treatment (10,11).
Alternate Names
2310008J16Rik; 2810405N18Rik; Anthrax toxin receptor 1; ANTR1; ANTXR cell adhesion molecule 1; ANTXR1; ATR; FLJ10601; FLJ11298; FLJ21776; GAPO; TEM8; Tumor endothelial marker 8
