Description
Product Usage Information
IHC Leica Bond: 1:50 - 1:200
Immunohistochemistry (Paraffin): 1:50
Storage
Supplied as liquid tissue culture supernatant containing sodium azide as a preservative. Stable for 6 months when stored at 4°C. Do not aliquot the antibody. Note: This product is only available in the format listed above. We are unable to provide this in a carrier free, custom formulation.
Protocol
Available protocols: IHC Leica Bond, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
IL3RA/CD123 (BR4MS) Mouse mAb recognizes endogenous levels of IL3RA/CD123 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a prokaryotic recombinant protein corresponding to 101 amino acids of the external domain of human IL3RA/CD123 protein.
Background
IL3RA/CD123 is the alpha subunit of an IL-3 receptor that also contains the common beta chain (βc) shared with receptors for other cytokines. IL3RA/CD123 alone binds to IL-3 with high specificity but low affinity and it does not transduce intracellular signaling, while βc alone does not bind to IL-3 appreciably. Together, IL3RA/CD123 and βc form the functional high-affinity receptor for IL-3. IL3RA/CD123 is mainly expressed on the hematopoietic progenitor cells, primarily the granulocytic and monocytic linages (1,2). IL3RA/CD123 is highly expressed on leukemic stem cells in the majority of AML (acute myeloid leukemia) patients and is correlated with poor prognosis. In addition, overexpression of IL3RA/CD123 has also been reported for other hematopoietic malignancies (3-5). It is considered as a valuable therapeutic target for these malignancies (6-8).
Alternate Names
CD123; CD123 antigen; hIL-3Ra; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA; IL3R; IL3RA; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor subunit alpha; interleukin 3 receptor, alpha (low affinity); Interleukin-3 receptor subunit alpha; MGC34174
