Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
PDE3A (F7I6O) Rabbit Monoclonal Antibody recognizes endogenous levels of total PDE3A protein. This antibody recognizes PDE3A1 and PDE3A2 isoforms but does not recognize PDE3A3 isoform.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val395 of human PDE3A1 protein.
Background
PDE3A is a member of the cyclic nucleotide phosphodiesterase (PDE) superfamily (1). The protein is a cAMP phosphodiesterase inhibited by cGMP. PDE3A regulates cAMP-activated signaling pathways through hydrolysis of cAMP (2). There are three isoforms of PDE3A: PDE3A1 (136 kDa), PDE3A2 (118 kDa), and PDE3A3 (94 kDa). They are generated by alternative splicing of the gene. All PDE3A isoforms share the same catalytic domain and promote cAMP hydrolysis in distinct but overlapped intracellular compartments (3). PDE3A has been shown to promote platelet aggregation, pulmonary thrombosis, and increased heart rate (4). The elevated phosphodiesterase activity from gain-of-function mutations in the gene leads to hypertension without cardiac damage (5). The compound DNMDP and synthetic molecular glue molecules promote PDE3A/Schlafen 12 (SLFN12) heterotetrameric complex formation, leading to a cytotoxic response in cancer cells that express high levels of these two proteins. Inducing this complex formation poses a potential therapeutic treatment in human cancer (6-8).
Alternate Names
cAMP phosphodiesterase, myocardial cGMP-inhibited; CGI-PDE; CGI-PDE A; CGI-PDE-A; cGMP-inhibited 3',5'-cyclic phosphodiesterase 3A; cGMP-inhibited 3',5'-cyclic phosphodiesterase A; cyclic GMP inhibited phosphodiesterase A; Cyclic GMP-inhibited phosphodiesterase A; HTNB; PDE3A; phosphodiesterase 3A; phosphodiesterase 3A, cGMP-inhibited
