Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
DLL3 (G93) Antibody detects transfected levels of DLL3. This antibody does not recognize transfected levels of rat DLL1 and human DLL4.
Species Reactivity: Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region surrounding residue Gly93 of mouse DLL3. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3, and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus, where it activates transcription. Notch ligands may also be processed in a way similar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6). Mutations in DLL3 cause Spondylocostal dysostoses (SCD), a diverse group of disorders of axial skeletal malformation (7-10).
Alternate Names
delta like canonical Notch ligand 3; delta-like 3; delta-like 3 (Drosophila); Delta-like protein 3; Delta3; DLL3; Drosophila Delta homolog 3; SCDO1
