Description
Product Usage Information
This product is the carrier free version of product #18880. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 18880
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
COL4A1 (E5M6R) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total COL4A1 protein. Non-specific diffuse cytoplasmic staining was observed in skeletal myocytes by immunohistochemistry.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp536 of human COL4A1 protein.
Background
Type IV collagens support extracellular matrix (ECM) function by forming a cross-linking network in the basement membrane zone. There are six different type IV collagens that are encoded by the following genes: , , , , , and . Among them, COL4A1 and COL4A2 are the most common collagens found in the basement membrane, and are usually used as a marker of the basement membrane dynamic stability (1). The type IV collagen fiber exists as a trimeric complex composed of two molecules of COL4A1 and one molecule of COL4A2. The N-terminal 7S domains of the protein function on intermolecular cross-linking, and C-terminal non-collagenous domains (NCI) are responsible for heterotrimer assembly. The trimeric fiber further associates to form a cross-linked sheet structure by NCI/NCI and 7S/7S interaction (2). COL4A1 plays a critical role in maintaining basement membrane integrity for normal tissue functions. Genetic mutations of COL4A1 and COL4A2 have been linked to cardiovascular disease, cerebral small vessel disease, and glomerulocystic kidney disease (3-5).
Alternate Names
Arresten; BSVD; BSVD1; CO4A1; COL4A1; COL4A1 NC1 domain; COL4A1s; Collagen 4; Collagen alpha-1(IV) chain; collagen IV; collagen IV, alpha-1 polypeptide; collagen of basement membrane, alpha-1 chain; collagen type IV; collagen type IV alpha 1 chain; collagen, type IV, alpha 1; PADMAL; RATOR; type IV collagen
