Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (Immunocytochemistry): 1:100 - 1:400
Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 67838 .
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
MAVS (D5A9E) Rabbit Monoclonal Antibody recognizes endogenous levels of total MAVS protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu202 of human MAVS protein.
Background
The mitochondrial antiviral signaling protein (MAVS, VISA) contributes to innate immunity by triggering IRF-3 and NF-κB activation in response to viral infection, leading to the production of IFN-β (1). The MAVS protein contains an N-terminal CARD domain and a C-terminal mitochondrial transmembrane domain. The MAVS adaptor protein plays a critical and specific role in viral defenses (2). MAVS acts downstream of the RIG-I RNA helicase and viral RNA sensor, leading to the recruitment of IKKε, TRIF, and TRAF6 (3,4). Some viruses have evolved strategies to circumvent these innate defenses by using proteases that cleave MAVS to prevent its mitochondrial localization (5,6).
Alternate Names
CARD adapter inducing interferon beta; CARD adapter inducing interferon-beta; CARD adaptor inducing IFN-beta; Cardif; DKFZp547C224; DKFZp666M015; FLJ27482; FLJ35386; FLJ38051; FLJ41962; IFN-B promoter stimulator 1; Interferon beta promoter stimulator protein 1; interferon-beta promoter stimulator protein 1; IPS-1; IPS1; KIAA1271; MAVS; MGC3260; mitochondrial anti-viral signaling protein; mitochondrial antiviral signaling protein; Mitochondrial antiviral-signaling protein; Putative NF-kappa-B-activating protein 031N; virus-induced signaling adapter variant 1b; virus-induced signaling adaptor; virus-induced signaling adaptor variant 1a; Virus-induced-signaling adapter; VISA
