Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
REDD1 Antibody detects transfected levels of REDD1 protein.
Species Reactivity: Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Asn60 of human REDD1. Antibodies are purified by peptide affinity chromatography.
Background
REDD1 ( gulated in evelopment and NA damage responses) expression is induced by hypoxia, cell stress, apoptosis and DNA damage. REDD1 is a transcriptional target of p53 and p63 following DNA damage, and links p63 to Ros (1). REDD1 functions as a negative regulator of mTOR in response to hypoxia in a tuberin-dependent manner (2). Depending on cell context, REDD1 can either be protecting or detrimental for cells under oxidative or ischemic stresses (3).
Alternate Names
DDIT4; Dig2; DNA damage inducible transcript 4; DNA damage-inducible transcript 4 protein; DNA-damage-inducible transcript 4; FLJ20500; HIF-1 responsive protein RTP801; HIF-1 responsive RTP801 (RTP801); Protein regulated in development and DNA damage response 1; REDD-1; REDD1; RP11-442H21.1; RTP801
