Description
Storage
Antibody beads supplied in IAP buffer containing 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: PTMScan
Background
Lysine formylation (For-K), also known as N -formyl-lysine, is a posttranslational modification (PTM) that occurs on the nitrogen atom of the lysine side chain. This PTM differs from N-terminal formylation that occurs in the context of initiation of prokaryotic translation and gramicidin production (1,2). Unlike other acyl-modifications on proteins, such as acetyl or succinyl lysine, formyl lysine is not primarily derived from an analogous coenzyme A metabolite. Instead, formyl lysine can be derived from diverse molecules, including peroxynitrite, 3'-formylphosphate, trichloroethylene oxide, and formaldehyde, and this PTM has been proposed as a biomarker for formaldehyde inhalation (3-7). Due to the various chemicals that can induce formylation, users should carefully consider their experimental workflows to avoid introducing spurious formyl lysines that can confound bona fide endogenous formyl lysine sites (8). Previous studies have identified formyl lysine sites on tau, histones, other non-histone chromatin factors, and milk proteins (3,9-11). In the context of tau, one proposed effect of this PTM is polymerization inhibition (3).
