Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
SUFU (C81H7) Rabbit Monoclonal Antibody detects endogenous level of total SUFU protein.
Species Reactivity: Human, Mouse, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala340 of human SUFU.
Background
SUFU (Suppressor of Fused) was identified in as a suppressor of the Fused (Fu) kinase that is essential for Hedgehog signaling during embryonic pattern formation (1). SUFU suppresses Hedgehog signaling by regulating the localization of the transcription factors Gli and Ci (2,3). In , SUFU may also positively regulate Hedgehog signaling depending on SUFU protein levels and Hedgehog signal intensity (4). SUFU may function as a tumor suppressor as inactivation and loss of heterozygosity of SUFU is associated with human rhabdomyosarcomas and medulloblastomas (5,6). Deletion of SUFU in mice results in embryonic lethality, while heterozygotes exhibit developmental defects characteristic of basal cell nevus syndrome. This aberrant developmental pathway is attributed to ligand-independent activation of Hedgehog signaling (7). GSK-3β binds and phosphorylates SUFU and additional information predicts that GSK-3β may positively regulate Hedgehog signaling through modification of SUFU (8).
Alternate Names
JBTS32; negative regulator of hedgehog signaling; PRO1280; SUFU; SUFU negative regulator of hedgehog signaling; SUFUH; SUFUXL; Suppressor of fused homolog; suppressor of fused homolog (Drosophila)
