Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
EGR3 Antibody recognizes endogenous levels of total EGR3 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe160 of human EGR3 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Early growth response 3 (EGR3) is a zinc finger transcription factor and one of four members of the early growth response family (1). Members of this family are basally expressed in the brain (2) and are immediate early response genes that are important for the induction of cellular programs of differentiation, proliferation, and cell death in response to environmental stimuli (1). EGR3 plays important roles in cellular growth and neuronal development (2) and an essential role in learning and memory processing of both short- and long-term hippocampus-dependent memory; it also mediates adaptation to stress and novelty (3). Research studies show that a lack of EGR3 results in abnormal neuronal and T cell development (4).
Alternate Names
early growth response 3; Early growth response protein 3; EGR-3; EGR3; MGC138484; PILOT; Zinc finger protein pilot
