Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
NUP98 (C39A3) Rabbit Monoclonal Antibody detects endogenous levels of total NUP98 protein. Reactivity by immunofluorescence is human only.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro671 of human NUP98.
Background
Nucleoporin 98 kDa (NUP98) is a component of the nuclear pore complex. It is expressed as three different precursors that undergo auto-cleavage to generate a common amino-terminal 98 kDa peptide (NUP98) and carboxy-terminal 6, 96 (NUP96) and 88 (p88) kDa peptides (1,2). NUP98 contains FG and GLFG repeat domains at its amino terminus and a RNA-binding domain in its carboxy terminus (3). The NUP98 gene is localized on chromosome 11p15.5, a region frequently rearranged in leukemias. To date, 15 fusion partners have been identified for NUP98 (4,5).
Alternate Names
96 kDa nucleoporin; 98 kDa nucleoporin; ADAR2; ADIR2; GLFG-repeat containing nucleoporin; Nuclear pore complex protein Nup96; Nuclear pore complex protein Nup98; Nuclear pore complex protein Nup98-Nup96; nucleoporin 96; nucleoporin 98 and 96 precursor; nucleoporin 98kD; nucleoporin 98kDa; Nucleoporin Nup96; Nucleoporin Nup98; NUP196; NUP96; NUP98; Nup98-96; Nup98-Nup96; NUP98/PHF23 fusion 2 protein
