Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Flow Cytometry (Fixed/Permeabilized): 1:1200 - 1:4800
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
SHMT1 (F2H4H) Rabbit Monoclonal Antibody recognizes endogenous levels of total SHMT1 protein. This antibody does not cross-react with SHMT2 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SHMT1 protein.
Background
Serine hydroxymethyltransferases 1 and 2 (SHMT1, SHMT2) are cytoplasmic and mitochondrial serine hydroxymethyltransferases, respectively (1,2). They catalyze the conversion of serine to glycine with the transfer of β-carbon from serine to tetrahydrofolate (THF) to form 5,10-methylene-THF (1,2). Research studies indicate that SHMT1 hemizygosity is associated with higher risk of intestinal cancer in mice of a certain genetic background (3). Suppression of SHMT2 was shown to block cell proliferation (4).
Alternate Names
14 kDa protein; CSHMT; cytoplasmic serine hydroxymethyltransferase; GLYC; Glycine hydroxymethyltransferase; MGC15229; MGC24556; serine hydroxymethyltransferase 1; serine hydroxymethyltransferase 1 (soluble); Serine hydroxymethyltransferase, cytosolic; Serine methylase; SHMT; SHMT1
