Description
Product Usage Information
Western Blotting: 1:1000
Immunofluorescence (Frozen): 1:100 - 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Frozen)
Specificity / Sensitivity
alpha-Synuclein Antibody detects endogenous levels of total synuclein protein. Non-specific labeling of cytoplasm in human cells and non-specific labeling of the mucosae in fixed frozen mouse colon may be observed by immunofluorescence.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of mouse synuclein. Antibodies are purified by peptide affinity chromatography.
Background
α-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. α-Synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Research studies have shown that mutations of the α-Synuclein gene are linked to Parkinson's disease (1).
Alternate Names
Alpha-synuclein; alpha-synuclein, isoform NACP140; I+/--synuclein; MGC110988; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; non-A4 component of amyloid; Non-A4 component of amyloid precursor; PARK1; PARK4; PD1; SNCA; synuclein alpha; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor); SYUA; truncated alpha synuclein
