Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:100
Immunofluorescence (Frozen): 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 79161 .
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen)
Specificity / Sensitivity
alpha/beta-Synuclein (Syn205) Mouse Monoclonal Antibody detects endogenous levels of total synuclein protein. This antibody detects recombinant α and β-synuclein (Giasson, B.I. et al., 2000).
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with human recombinant α- and β-synucleins and the epitope maps to the carboxy terminus of the protein.
Background
α-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. α-Synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Research studies have shown that mutations of the α-Synuclein gene are linked to Parkinson's disease (1).
Alternate Names
Alpha-synuclein; alpha-synuclein, isoform NACP140; Beta-synuclein; I(2)-synuclein; I+/--synuclein; MGC110988; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; non-A4 component of amyloid; Non-A4 component of amyloid precursor; PARK1; PARK4; PD1; SNCA; SNCB; synuclein alpha; synuclein alpha-140; synuclein beta; synuclein, alpha (non A4 component of amyloid precursor); synuclein, beta; SYUA; SYUB; truncated alpha synuclein
