Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
SARS-CoV-1 Spike Protein (E7C5Y) Rabbit Monoclonal Antibody recognizes transfected levels of total SARS-CoV-1 spike protein. This antibody does not cross-react with spike proteins from SARS-CoV-2 or MERS coronaviruses.
Species Reactivity: Virus
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of SARS-CoV-1 spike protein.
Background
The cause of the SARS epidemic in 2003 was a novel pathogenic coronavirus, originally termed SARS-CoV (severe acute respiratory syndrome coronavirus) (1). Following the 2019 emergence of SARS-CoV-2 and the COVID-19 pandemic, SARS-CoV is now often referred to as SARS-CoV-1. SARS-CoV-1 is a member of the Coronaviridae family of viruses (2). The genome of SARS-CoV-1 is similar to other coronaviruses, and is comprised of four key structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (3). Coronavirus spike proteins are class I fusion proteins that harbor an ectodomain, a transmembrane domain, and an intracellular tail (4,5). The highly glycosylated ectodomain projects from the viral envelope surface and facilitates attachment and fusion with the host cell plasma membrane. The ectodomain can be further subdivided into host receptor-binding domain (RBD) (S1) and membrane-fusion (S2) subunits, which are produced upon proteolysis by host proteases at the S1/S2 junction, with possible secondary cleavage occurring at a downstream site (S2/S2') (6). S1 and S2 subunits remain associated after cleavage and assemble into crown-like homotrimers (3,5). As with SARS-CoV-2, the SARS-CoV-1 spike protein utilizes the angiotensin-converting enzyme 2 (ACE2) protein as a receptor for cellular entry (7-9). Spike protein subunits represent a key antigenic feature of coronavirus virions, and therefore represent an important target of vaccines, novel therapeutic antibodies, and small-molecule inhibitors (10,11).
Alternate Names
E2; E2 glycoprotein precursor; Peplomer protein; S glycoprotein; SARS-CoV spike protein; sars2; spike glycoprotein; SPIKE_CVHSA
