Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Phospho-PEA-15 (Ser104) Antibody detects endogenous levels of PEA-15 only when phosphorylated at serine 104.
Species Reactivity: Human, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser104 of human PEA-15. Antibodies are purified by protein A and peptide affinity chromatography.
Background
PEA-15 is a 15 kDa phosphoprotein expressed abundantly in astrocytes and fibroblasts as well as in tissues, including the lung and eye (1). The protein has been shown to coordinate cell growth, death, and glucose utilization (2-4). The amino-terminal DED domain of PEA-15 mediates its binding to FADD or Erk and further regulates the Erk and apoptosis signaling pathways. PEA-15 can be phosphorylated at two serine residues, Ser104 and Ser116, located within the carboxy terminus. Phosphorylation at these sites regulates binding to Erk and FADD (2,3).
Alternate Names
15 kDa phosphoprotein enriched in astrocytes; Astrocytic phosphoprotein PEA-15; HMAT1; homolog of mouse MAT-1 oncogene; HUMMAT1H; mammary transforming gene 1, mouse, homolog of; MAT1; MAT1H; PEA-15; PEA15; PED; PED-PEA15; PED/PEA15; phosphoprotein enriched in astrocytes 15; Phosphoprotein enriched in astrocytes, 15kD; Phosphoprotein enriched in diabetes; proliferation and apoptosis adaptor 15; proliferation and apoptosis adaptor protein 15
