Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Nectin-1/PVRL1 (F7J9O) Rabbit Monoclonal Antibody recognizes endogenous levels of total nectin-1/PVRL1 protein.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln478 of human nectin-1/PVRL1 protein.
Background
Nectin cell adhesion molecule 1 (Nectin-1), also known as poliovirus receptor-related 1 (PVRL1) and CD111, is a single-pass type I membrane glycoprotein and is part of the immunoglobulin (Ig) superfamily of cell adhesion proteins (1). Nectins are composed of an ectodomain containing three tandem Ig-like regions arranged as an N-terminal variable-like (V) domain followed by two constant-like (C) domains, a single transmembrane region, and a cytoplasmic domain that binds to the actin cytoskeleton through the adaptor protein afadin (2). Nectin-1 plays important roles in adherens junction (AJ) formation in epithelial cells and neuronal synapses (1,3). Like other nectin family members, nectin-1 is used as an entry receptor for several mammalian viruses, including herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), porcine pseudorabies virus (PRV), bovine herpes virus type 1 (BHV-1), and simian B virus (4-6). Nectin-1 also plays a role in the immune system, where it interacts with CD96 expressed on natural killer (NK) cells and T cell subsets, and modulates cancer cell and virally infected cell clearance (7,8).  Â
Alternate Names
CD111; CLPED1; ectodermal dysplasia 4 (Margarita Island type); ED4; herpes simplex virus type 1 sensitivity; Herpes virus entry mediator C; Herpesvirus entry mediator C; Herpesvirus Ig-like receptor; HIgR; HV1S; HVEC; MGC142031; MGC16207; NECT1; nectin 1; Nectin cell adhesion molecule 1; Nectin-1; NECTIN1; OFC7; poliovirus receptor-like 1; poliovirus receptor-related 1 (herpesvirus entry mediator C); Poliovirus receptor-related protein 1; PRR; PRR1; PVRL1; PVRR; PVRR1; SK-12
