Description
Product Usage Information
Western Blotting: 1:1000
Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 24152 .
Protocol
Available protocols: Western Blotting, Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
Phospho-Bcl-2 (Ser70) (5H2) Rabbit Monoclonal Antibody detects endogenous levels of Bcl-2 only when phosphorylated at serine 70. This antibody does not cross-react with nonphosphorylated Bcl-2 at endogenous levels or with other Bcl-2 family members.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding serine 70 of human Bcl-2.
Background
Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2, including Thr56, Ser70, Thr74, and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway and that phosphorylation of Bcl-2 may be a marker for mitotic events (4,5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin-3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7).
Alternate Names
Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl-2; BCL2; BCL2 apoptosis regulator; PPP1R50; protein phosphatase 1, regulatory subunit 50
