Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
IRX3 (F7S2F) Rabbit Monoclonal Antibody recognizes endogenous levels of total IRX3 protein. This antibody cross-reacts with a 15 kDa protein of unknown identity in some cell extracts.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro485 of human IRX3 protein.
Background
The Iroquois-class homeodomain proteins (IRX1-6) comprise a family of transcription factors, initially identified in a mutagenesis experiment in designed to identify genes involved in embryonic patterning (1,2). Members of the Iroquois-class protein family contain a three amino acid loop extension (TALE) homeodomain and an IRO sequence motif of unknown function that is unique to this protein family, but which bears similarity to Notch receptor domains involved in protein-protein interactions (3,4). Research studies in various animal models suggest important roles for IRX proteins in early development, including neural patterning, heart field specification, kidney development, and lung development (5-8). Moreover, studies in mice have shown an essential role for IRX proteins in eye development, where they are required for the specification of ommatidia and photoreceptor cells (9). IRX3 is expressed during nervous system, renal, and cardiac development and controls genetic programs that guide development of these organs (10). Research studies have demonstrated that mice lacking both Irx3 and its paralog, Irx5, die in utero due, in part, to cardiac defects (11). In the context of human disease, research studies have demonstrated that IRX3 is aberrantly overexpressed and oncogenic in multiple types of human hematologic malignancies, including acute myeloid leukemia (AML) (12,13).
Alternate Names
FLJ99187; Homeodomain protein IRXB1; iroquois homeobox 3; Iroquois homeobox protein 3; iroquois-class homeodomain protein IRX-1; Iroquois-class homeodomain protein IRX-3; IRX-1; IRX3; IRXB1
