Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
hnRNP M Antibody recognizes endogenous levels of total hnRNP M protein.
Species Reactivity: Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro43 of human hnRNP M protein. Antibodies are purified by peptide affinity chromatography.
Background
Heterogenous nuclear ribonucleoprotein M (hnRNP M) belongs to a family of RNA-binding multi-protein complexes (hnRNP proteins) that facilitate pre-mRNA processing (1,2). hnRNP M binds to GU rich regions of RNA and exists as 4 isoforms named M1-M4 (2). Binding to pre-mRNA in early stages of spliceosome assembly, hnRNP M contributes to splicing events by inducing exon skipping as well as promoting exon inclusion (3,4). hnRNP M is expressed on the surface of Kupffer cells, and acts as a receptor for carcinoembryonic antigen (CEA), a biomarker for colorectal cancer metastasis to the liver (5-7). hnRNP M has also shown to be a key regulator of the innate immune response in macrophages, where it represses key inflammatory and antimicrobial genes (8,9).
Alternate Names
CEA receptor; CEAR; DKFZp547H118; Heterogeneous nuclear ribonucleoprotein M; heterogenous nuclear ribonucleoprotein M; heterogenous nuclear ribonucleoprotein M4; hnRNA-binding protein M4; hnRNP M; HNRNPM; HNRNPM4; HNRPM; HNRPM4; HTGR1; M4 protein; N-acetylglucosamine receptor 1; NAGR1
