Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
CPEB4 Antibody recognizes endogenous levels of total CPEB4 protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CPEB4 protein. Antibodies are purified by peptide affinity chromatography.
Background
Cytoplasmic polyadenylation element (CPE)-binding (CPEB) proteins are a family of sequence-specific RNA-binding proteins that bind to the CPE, a uridine-rich sequence element within the mRNA 3' UTR, to regulate mRNA translation (1). The CPEB family consists of four members in vertebrates: CPEB1, CPEB2, CPEB3, and CPEB4. All four paralogs share sequence similarities in their carboxy-terminal RNA-binding domains, but vary in the sequence of their amino-terminal regulatory domains (2). CPEB4 has specifically been linked to autism spectrum disorder (ASD), where it has been found to bind to the mRNA transcripts of most high-confidence ASD risk genes (3). Moreover, young idiopathic ASD individuals show decreased CPEB4 protein levels in the brain, and consequently decreased protein levels of multiple ASD risk gene transcripts compared to controls (3). CPEB4 is also abnormally expressed in a large number of cancers and often correlates with poor prognosis, including glioblastoma/glioma, head and neck squamous cell carcinoma, non-small cell lung cancer, colon cancer, and breast cancer, among others (4-9).
Alternate Names
CPE-binding protein 4; CPE-BP4; CPEB4; cytoplasmic polyadenylation element binding protein 4; Cytoplasmic polyadenylation element-binding protein 4; hCPEB-4; KIAA1673
