Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
ERp57 (G117) Antibody detects endogenous levels of total ERp57 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly117 of human ERp57. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Secretory proteins translocate into the endoplasmic reticulum (ER) after their synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds (2). Other ER resident proteins that possess the thioredoxin homology domains, including endoplasmic reticulum stress protein 57 (ERp57), constitute the PDI family (2). ERp57 interacts with calnexin and calreticulin (3) and is suggested to play a role in the isomerization of disulfide bonds on certain glycoproteins (3).
Alternate Names
58 kDa glucose-regulated protein; 58 kDa microsomal protein; Disulfide isomerase ER-60; endoplasmic reticulum P58; Endoplasmic reticulum resident protein 57; Endoplasmic reticulum resident protein 60; epididymis secretory protein Li 269; epididymis secretory sperm binding protein Li 93n; ER protein 57; ER protein 60; ER60; ERP57; ERP60; ERp61; glucose regulated protein, 58kDa; GRP57; GRP58; HEL-S-269; HEL-S-93n; HsT17083; p58; PDIA3; phospholipase C-alpha; PI-PLC; protein disulfide isomerase family A member 3; protein disulfide isomerase family A, member 3; protein disulfide isomerase-associated 3; Protein disulfide-isomerase A3
