Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:200
Immunohistochemistry (Paraffin): 1:400 - 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
AMACR (F7T6V) Rabbit Monoclonal Antibody recognizes endogenous levels of total AMACR protein. Non-specific diffuse staining was observed in human skeletal muscle, breast myoepithelium, and scattered cells in thymus by immunohistochemistry.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human AMACR protein.
Background
α-Methylacyl-CoA racemase (AMACR), an enzyme localized in peroxisomes and mitochondria, is involved in the β-oxidation of branched-chain fatty acids and fatty acid derivatives (1). AMACR has been reported to be a biomarker for prostate cancer (2-4). AMACR expression is also related to other types of cancers, such as hepatocellular carcinoma (1), noninvasive bladder cancer (5), colorectal cancer (6), and gastric adenocarcinoma (7).
Alternate Names
2-methylacyl-CoA racemase; Alpha-methylacyl-CoA racemase; AMACR; AMACRD; CBAS4; P504S; RACE; RM
