Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Erlin-1 Antibody detects endogenous levels of total human erlin-1 protein.
Species Reactivity: Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly320 of human erlin-1. Antibodies are purified by peptide affinity chromatography.
Background
Erlin-1 and erlin-2 (SPFH1 and SPFH2) are SPFH domain-containing proteins that belong to the prohibitin family (1,2). The N-termal domain of erlin proteins contains ER-targeting sequences responsible for their translocation to the endoplasmic reticulum (ER) (3). In the ER, erlin-1 and erlin-2 specifically associate with the detergent resistant lipid raft microdomain of the membrane (3). Erlin-1 may be involved in dentritic cell activation (4) and erlin-2 has been shown to regulate the ER-associated degradation (ERAD) pathway by interacting with endogenous substrates and resulting in their polyubiquitination and degradation (5).
Alternate Names
Band_7 23-211 Keo4 (Interim) similar to C.elegans protein C42C1.9; C10orf69; Endoplasmic reticulum lipid raft-associated protein 1; ER lipid raft associated 1; Erlin-1; ERLIN1; ERLN1; KE04; KEO4; Protein KE04; SPFH domain family, member 1; SPFH domain-containing protein 1; SPFH1; SPG62; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 1
