Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Erlin-2 Antibody detects endogenous levels of human erlin-2 protein.
Species Reactivity: Human, Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln315 of mouse erlin-2. Antibodies are purified by peptide affinity chromatography.
Background
Erlin-1 and erlin-2 (SPFH1 and SPFH2) are SPFH domain-containing proteins that belong to the prohibitin family (1,2). The N-termal domain of erlin proteins contains ER-targeting sequences responsible for their translocation to the endoplasmic reticulum (ER) (3). In the ER, erlin-1 and erlin-2 specifically associate with the detergent resistant lipid raft microdomain of the membrane (3). Erlin-1 may be involved in dentritic cell activation (4) and erlin-2 has been shown to regulate the ER-associated degradation (ERAD) pathway by interacting with endogenous substrates and resulting in their polyubiquitination and degradation (5).
Alternate Names
C8orf2; Endoplasmic reticulum lipid raft-associated protein 2; epididymis secretory sperm binding protein; ER lipid raft associated 2; Erlin-2; ERLIN2; ERLN2; MGC87072; NET32; spastic paraplegia 18 (autosomal dominant); SPFH domain family, member 2; SPFH domain-containing protein 2; SPFH2; SPG18; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2
