Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
RPL15 (E3X8M) Rabbit Monoclonal Antibody recognizes endogenous levels of total RPL15 protein. This antibody detects a nonspecific signal around 34 kDa in lysates of some cell lines.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RPL15 protein.
Background
Ribosomal protein L15 (RPL15) is a structural component of the large 60S subunit of the ribosome. Studies show that overexpressed RPL15 protein promotes metastatic growth of breast cancer patient-derived circulating tumor cells. Overexpression of RPL15 was shown to increase translation of other ribosomal proteins of large and small subunits and cell cycle regulators (1). Furthermore, the DNA topoisomerase I inhibitor topotecan binds to RPL15 and suppresses preribosomal subunit formation, which causes ribosomal stress and enhances DNA topoisomerase I-independent antitumor immune activation mediated by damage-associated molecular patterns (2). In addition, RPL15 expression is upregulated in colon cancer cells. RPL15 deficiency leads to ribosomal stress and, therefore, apoptosis of colon cancer cells (3).
Alternate Names
60S ribosomal protein L15; DBA12; EC45; FLJ26304; L15; Large ribosomal subunit protein eL15; MGC88603; ribosomal protein L15; RL15; RPL10; RPL15; RPLY10; RPYL10
