Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
MAPKAPK-3 Antibody detects endogenous levels of total MAPKAPK-3 protein. This antibody does not cross-react with MAPKAPK-2 or -5.
Species Reactivity: Human, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal region of human MAPKAPK-3. Antibodies are purified by protein A and peptide affinity chromatography.
Background
MAPKAPK-3 has a single potential SH3-binding site in the proline-rich amino terminus, a putative ATP-binding site, 2 MAP kinase phosphorylation site motifs, and a putative nuclear localization signal. It shares 72% nucleotide and 75% amino acid identity with MAPKAPK-2 (1). MAPKAPK-3 has been shown to be activated by growth inducers and stress stimulation of cells. studies have demonstrated that Erk, p38 MAP kinase, and Jun amino-terminal kinase are able to phosphorylate and activate MAPKAPK-3, which suggested a role for this kinase as an integrative element of signaling in both mitogen and stress responses (2). MAPKAPK-3 was reported to interact with, phosphorylate, and repress the activity of E47, which is a basic helix-loop-helix transcription factor involved in the regulation of tissue-specific gene expression and cell differentiation (3). MAPKAPK-3 may also support luteal maturation through the phosphorylation and activation of the nuclear transcription factor CREB (4).
Alternate Names
3pK; Chromosome 3p kinase; MAP kinase-activated protein kinase 3; MAPK activated protein kinase 3; MAPK-activated protein kinase 3; MAPK3; MAPKAP kinase 3; MAPKAP-K3; MAPKAP3; MAPKAPK-3; MAPKAPK3; MDPT3; mitogen-activated protein kinase-activated protein kinase 3; MK-3; MK3
