Description
Product Usage Information
Western Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:250 - 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
Respiratory Syncytial Virus (RSV) Nucleoprotein (N) (F5S9J) Rabbit Monoclonal Antibody recognizes endogenous levels of total RSV nucleoprotein in RSV-infected cells and tissue. Based on sequence homology, this antibody will broadly detect RSV nucleoprotein subtypes and species variants.
Species Reactivity: Virus
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro341 of human respiratory syncytial virus A (strain A2) nucleoprotein (UniProt #P03418).
Background
The respiratory syncytial virus (RSV), also known as human orthopneumovirus, is a negative-sense, single-stranded RNA virus that causes serious acute respiratory disease, primarily in infants and older adults. The RSV genome consists of 10 genes encoding 11 proteins. NS1 and NS2 are nonstructural proteins. Structural proteins are matrix (M), small hydrophobic (SH), attachment glycoprotein (G), fusion glycoprotein (F), transcriptional processivity factor (M2-1), RNA regulatory factor (M2-2), nucleoprotein (N), phosphoprotein (P), and large polymerase (L). The nucleocapsid is surrounded by a host-derived lipid envelope with protruding viral glycoproteins to form the virion (1,2). N coats and stabilizes the viral RNA, and is required for replication (3). Therapeutics targeting several viral proteins are in development, including monoclonal antibodies targeting F and G proteins, and small-molecule inhibitors targeting N and L proteins (4).
Alternate Names
nucleocapsid protein; Nucleoprotein (N); protein N
