Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:200
Immunofluorescence (Frozen): 1:100 - 1:400
Immunofluorescence (Immunocytochemistry): 1:800 - 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
MAFB (E3O9X) Rabbit Monoclonal Antibody recognizes endogenous levels of total MAFB protein. This antibody does not cross-react with MAFA.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro42 of human MAFB protein.
Background
MAFB belongs to the musculoaponeurotic fibrosarcoma (MAF) family of basic leucine-zipper transcription factors (1). In mouse embryo, MAFB expression is first detected at E10.5 (2,3). Early in development, MAFB drives differentiation of both glucagon-producing α-cells and insulin-producing β-cells in the pancreas, but later plays a more decisive role in the maturation and maintenance of functional α-cells (4,5). Consistent with MAFB playing a critical role in mature α-cells, MAFB is enriched in α-cells within two weeks of birth in the pancreas (6). Glucagon and insulin secretion is tightly regulated, and imbalances in these hormones contribute to metabolic conditions. Therefore, understanding the role of MAFB in α-cell development, maintenance, and physiological function may contribute to developing deeper insights into how these cells contribute to metabolic diseases like diabetes. MAFB also regulates monocyte differentiation, indicating MAFB functions beyond the pancreas (7).
Alternate Names
DURS3; Kreisler maf-related leucine zipper homolog; KRML; MAF bZIP transcription factor B; Maf-B; MAFB; MAFB/Kreisler basic region/leucine zipper transcription factor; MCTO; MGC43127; Transcription factor MafB; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog B; V-maf musculoaponeurotic fibrosarcoma oncogene homolog B; v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian)
