Description
Product Usage Information
Western Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:100 - 1:400
Immunofluorescence (Immunocytochemistry): 1:1500 - 1:6000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 31059 .
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
HNF-4 alpha (C11F12) Rabbit Monoclonal Antibody detects endogenous levels of total HNF4α protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser461 of human HNF4α.
Background
Hepatocyte nuclear factor 4α (HNF4α) is a transcription factor that belongs to the steroid hormone receptor superfamily and is enriched in liver (1). HNF4α, in association with PGC-1α, activates gluconeogenic genes such as phosphoenolpyruvate carboxykinase and glucose-6-phosphatase genes in fasted livers (2,3). Conditional knockout of the HNF4α gene in the mouse liver destroys lipid homeostasis and leads to lipid accumulation in the liver and a reduction of serum cholesterol and triglyceride levels (4). Mutations in HNF4α have been linked to maturity-onset diabetes of the young (MODY) (5).
Alternate Names
FLJ39654; FRTS4; hepatic nuclear factor 4 alpha; hepatocyte nuclear factor 4 alpha; Hepatocyte nuclear factor 4-alpha; hepatocyte nuclear factor 4, alpha; HNF-4-alpha; HNF4; HNF4 alpha; HNF4A; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; HNF4alpha10/11/12; MODY; MODY1; NR2A1; NR2A21; Nuclear receptor subfamily 2 group A member 1; TCF; TCF-14; TCF14; Transcription factor 14; Transcription factor HNF-4; transcription factor-14
