Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
IFN-alpha (8C21) Mouse Monoclonal Antibody detects recombinant human interferon-α protein. This antibody does not cross-react with human interferon-β and -γ.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with purified natural human interferon-α proteins.
Background
Interferons (IFNs) appear both locally and systematically early after viral infection and participate in limiting the spread of infection. They also affect cell differentiation, growth, surface antigen expression, and immunoregulation (1). There are three naturally occurring interferons: α, β, and γ. IFN-α is derived from lymphoblastic tissue and has a number of therapeutic applications in the treatment of various human cancers and diseases of viral origin. Recombinant IFN-α from both natural and synthetic genes binds to a common cell surface receptor and induces antiviral activity in a variety of cell lines. When binding to discrete cell surface receptors on target cells, IFN-α induces rapid changes in Jak/Stat phosphorylation, which initiates the Jak/Stat signaling pathway (2). IFN-α signaling also involves production of DAG without an increased intracellular free calcium concentration and the subsequent activation of calcium-independent isoforms of PKC (β and ε) (3). All IFN-α signaling pathways lead to final alterations of gene expression, which mediate their pleiotropic biologic activities.
Alternate Names
IFL; IFN; IFN-ALPHA; IFN-alpha-1; IFN-alphaD; IFNA@; IFNA1; interferon alpha 1; interferon alpha 1b; Interferon alpha-1; Interferon alpha-D; interferon-alpha1; LeIF D
