Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:50 - 1:250
Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:800
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 23689 .
Protocol
Available protocols: Western Blotting, Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
IL-1 beta (D6D6T) Rabbit Monoclonal Antibody recognizes endogenous levels of total IL-1β protein.
Species Reactivity: Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to mouse IL-1β protein.
Background
Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity.
Alternate Names
IL-; IL-1 beta; Il-1b; IL-1beta; Il1b; interleukin 1 beta; Interleukin-1 beta; OTTMUSP00000016525
