Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
LHPP (F4I8S) Rabbit Monoclonal Antibody recognizes endogenous levels of total LHPP protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu257 of human LHPP protein.
Background
Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP) is a highly evolutionarily conserved histidine phosphatase (1). The enzyme hydrolyzes oxygen-phosphorus bonds in inorganic pyrophosphate and nitrogen-phosphorus bonds in phospholysine, phosphohistidine, and imidodiphosphate (2). LHPP expression is downregulated in multiple cancer types (3). The protein acts as a tumor suppressor, inhibiting proliferation, migration, and invasion and promoting apoptosis during cancer development (4), probably through its inhibitory effect on Akt and TGF-β/SMAD pathway signaling (5-8). The gene has been linked to major depressive disorder (MDD), also known as clinical depression (9). In the lysosome, LHPP mediates inorganic pyrophosphate (PPi) hydrolysis (i.e., PPi to Pi) to facilitate vacuolar ATPase (V-ATPase)-dependent proton transport across the lysosomal membrane. Under stress conditions, LHPP activity maintains an acidic pH environment in the lysosome to promote C/EBPβ degradation, leading to reduced cytokine release and resulting in neurodepression (10).
Alternate Names
FLJ44846; FLJ46044; HDHD2B; hLHPP; LHPP; MGC117251; MGC142189; MGC142191; Phospholysine phosphohistidine inorganic pyrophosphate phosphatase
