Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
TAB1 (C25E9) Rabbit Monoclonal Antibody detects endogenous levels of total TAB1 protein.
Species Reactivity: Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro381 of human TAB1.
Background
TAK1 is a mitogen-activated protein kinase kinase kinase activated by TGF-β and various pro-inflammatory signals (1,2). , TAK1 activation requires its association with TAK1 binding protein 1 (TAB1), which triggers TAK1 autophosphorylation at Thr184 and Thr187 (3,4). The TAB2 adaptor protein links TAK1 with TRAF6 to mediate TAK1 activation following IL-1 stimulation (5). Once activated, TAK1 phosphorylates the MAPK kinases MKK4 and MKK3/6, which activate JNK and p38 MAPK, respectively. TAK1 and TRAF6 also activate the NF-κB pathway by phosphorylating the NF-κB inducing kinase (NIK) to trigger subsequent activation of IKK (2,6). In addition to TAK1, TAB1 interacts with and activates p38α MAPK (7). Targeted disruption of the TAB1 gene in mice causes a drastic reduction in TAK1 activity and leads to embryonic lethality (8).
Alternate Names
3'-Tab1; MAP3K7IP1; MGC57664; mitogen-activated protein kinase kinase kinase 7 interacting protein 1; Mitogen-activated protein kinase kinase kinase 7-interacting protein 1; TAB1; TAK1-binding protein 1; TGF-beta activated kinase 1 (MAP3K7) binding protein 1; TGF-beta activated kinase 1/MAP3K7 binding protein 1; TGF-beta-activated kinase 1 and MAP3K7-binding protein 1; TGF-beta-activated kinase 1-binding protein 1; transforming growth factor beta-activated kinase-binding protein 1
