Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Phospho-Gab1 (Tyr627) (C32H2) Rabbit Monoclonal Antibody detects endogenous levels of Gab1 only when phosphorylated at Tyr627. This antibody may cross-react with phosphorylated Gab2 or Gab3, or with activated receptor tyrosine kinases (RTKs).
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr627 of human Gab1.
Background
The Grb-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ (8). Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2 (6,9).
Alternate Names
deafness, autosomal recessive 26; DFNB26; GAB1; GRB2 associated binding protein 1; GRB2-associated binder 1; GRB2-associated binding protein 1; GRB2-associated-binding protein 1; Growth factor receptor bound protein 2-associated protein 1; symbol withdrawn, see GAB1
