Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
CaMKI-delta Antibody detects endogenous levels of total CamKI-δ protein. This antibody may detect other isoforms of CaMKI.
Species Reactivity: Human, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human CaMKI-δ. Antibodies are purified by peptide affinity chromatography.
Background
The Ca /calmodulin-dependent kinase (CaMK) family, which is activated in response to elevation of intracellular Ca , includes CaMKI, CaMKII, CaMKIV, and CaMK-kinases (CaMKKs) (1,2). CaMKI is a downstream substrate of CaMKK and has four isoforms: CaMKI-α, CaMKI-β, CaMKI-γ, and CaMKI-δ. CaMKI is present in most cell types and may be involved in cellular functions, including transcription, cytoskeletal organization, axonal growth cone motility, and long-term potentiation in neurons (3-6). CaMKII is also ubiquitously expressed in most cell types. While muscular CaMKII has been linked to activation of mitochondrial biogenesis in muscle hypertrophy response, neuronal CaMKII regulates important neuronal functions, including neurotransmitter synthesis, neurotransmitter release, modulation of ion channel activity, cellular transport, cell morphology and neurite extension, synaptic plasticity, learning and memory, and gene expression (7). Like CaMKI, CaMKIV is also a substrate of CaMKKs and is primarily restricted to the nucleus of neurons. CaMKIV regulates gene transcription in neurons through phosphorylation of transcription factors such as CREB and is required for fear memory (8). CaMKI-δ translocates to the nucleus upon intracellular Ca influx and is activated through phosphorylation of Thr180 by CaMKK (9).
Alternate Names
calcium/calmodulin dependent protein kinase ID; calcium/calmodulin-dependent protein kinase ID; Calcium/calmodulin-dependent protein kinase type 1D; CaM kinase I delta; CaM kinase ID; CaM-K1; CaM-KI delta; CAMK1D; CAMK1D/ANAPC5 fusion; CaMKI delta; CaMKI-like protein kinase; CaMKID; CKLiK; KCC1D
