Description
Product Usage Information
Immunofluorescence (Immunocytochemistry): 1:50 - 1:100
Flow Cytometry (Fixed/Permeabilized): 1:50
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Available protocols: Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
GST-Tag (26H1) Mouse Monoclonal Antibody (Alexa Fluor
Species Reactivity: All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a GST fusion protein. The antibody was conjugated to Alexa Fluor 488 under optimal conditions with an F/P ratio 2-6.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).
