Description
Product Usage Information
Western Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:400 - 1:1600
Immunofluorescence (Immunocytochemistry): 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 10130 .
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
GSTP1 (3F2) Mouse Monoclonal Antibody detects endogenous levels of total GSTP1 protein. This antibody does not cross react with GST-tagged proteins.
Species Reactivity: Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant GSTP1 protein.
Background
Glutathione S-transferases (GSTs) are a family of isoenzymes that detoxify electrophiles through conjugation to thiol-reduced glutathione (GSH). Thus, they are critical in protecting cells from toxins (drugs, pesticides, carcinogens) and oxidative stress (1). Eight isoforms of cytosolic-soluble GSTs (α, κ, μ, Ï, Ï, θ, ζ, and Ï) are identified, while only GST-α, -μ, and -Ï are described in the central nervous system (2). GSTP1 (GSTÏ) is overexpressed in early stages of carcinogenesis and can be used as a neoplastic marker in tumor tissues (3). GSTP1 directly inhibits TRAF2 and JNK but not NF-κB (4,5). Corresponding GSTP1 gene polymorphisms affect substrate selectivity and stability, and the oxidative milieu in dopaminergic neurons, which increases the susceptibility to Parkinson's disease (6).
Alternate Names
deafness, X-linked 7; DFN7; epididymis secretory protein Li 22; FAEES3; fatty acid ethyl ester synthase III; Glutathione S-transferase P; glutathione S-transferase pi 1; GST class-pi; GST3; GSTP; GSTP1; GSTP1-1; HEL-S-22; PI
