Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
PHD-1/Egln2 (F7T8H) Rabbit Monoclonal Antibody recognizes endogenous levels of total PHD-1/Egln2 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to human PHD-1/Egln2 protein.
Background
PHD-1 (Egln2), PHD-2 (Egln1), and PHD-3 (Egln3) are members of the Egln family of proline hydroxylases. They function as oxygen sensors that catalyze the hydroxylation of HIF on prolines 564 and 402, initiating the first step of HIF degradation through the VHL/ubiquitin pathway (1,2). PHD-1 is highly expressed in a wide array of tissues, whereas PHD-2 and PHD-3 are expressed mainly in heart and skeletal muscle (1,3). The mRNA levels of PHD are upregulated by HIF through the hypoxia-response element under low oxygen conditions (4-7). These three enzymes also exhibit different peptide specificity target proteins, PHD-1 and PHD-2 can hydroxylate both proline 402 and proline 564, but PHD-3 can only hydroxylate proline 564 (2,8). In addition to HIF, PHD enzymes have also has been shown to catalyze the hydroxylation of RNA polymerase subunits and myogenin (3,9).
Alternate Names
DKFZp434E026; Egl nine homolog 2; egl nine homolog 2 (C. elegans); egl-9 family hypoxia inducible factor 2; EGLN2; EIT-6; EIT6; Estrogen-induced tag 6; HIF prolyl hydroxylase 1; HIF-PH1; HIF-prolyl hydroxylase 1; HIFPH1; HPH-1; HPH-3; Hypoxia-inducible factor prolyl hydroxylase 1; PHD1; Prolyl hydroxylase domain-containing protein 1; Prolyl hydroxylase EGLN2
