Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Immunofluorescence (Frozen): 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen)
Specificity / Sensitivity
Myosin Va Antibody detects endogenous levels of total myosin Va heavy chain. Based on sequence homology, this antibody is expected to detect all known myosin Va splice variants.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human myosin Va.
Background
Myosin Va is a molecular motor protein involved in the transport of organelles, vesicles and other cellular cargo along actin filaments (reviewed in 1). The molecule consists of two identical heavy chains, which dimerize via helical domains in a coiled coil structure. The amino-terminal motor domains of the heavy chains contain both the ATPase and the actin-binding activities of myosin Va. The globular tail domains act in a regulatory capacity, binding the myosin Va cargo (2) or inhibiting motor activity by binding the head domains and preventing ATP consumption (3). Mutation of the murine dilute gene, which encodes myosin Va, causes defects in coat pigmentation as well as severe neurological defects (4). In melanocytes, the coiled coil structure of myosin Va is important in regulating the trafficking of melanosomes in conjunction with melanophilin and Rab27a (5). Myosin Va regulates trafficking and exocytosis of secretory granules in neuroendocrine cells (reviewed in 6) as well as RNA transport and distribution (7).
Alternate Names
9630007J19Rik; AI413174; AI661011; d; d-120J; Dbv; Dilute; dilute lethal-20J protein; Dilute myosin heavy chain, non-muscle; flail; flailer; flr; MVa; Myo; Myo5; Myo5a; myosin VA; Myosin-Va; MyoVA; Sev-1; Unconventional myosin-Va
